Gel Imager and Gel Documentation Features, Styles, and Applications Comparison
什么是凝胶成像仪？（凝胶文件系统）
Laboratory gel imagers或GEL文档系统由研究实验室用于可视化和通过凝胶电泳分离的光学文献核酸样品，计数微生物菌落，蛋白质印迹中的单独蛋白质样品，并通过薄层色谱（TLC）鉴定混合物。
How Does a Gel Imager or Gel Documentation System Work?
Gel imagers, or gel docs, contain an ultraviolet (UV) or visible (blue or white) transilluminator, a hood to block the samples from external light sources and a high-resolution camera for image capture.
凝胶成像程序程序
The samples, loaded onto agarose gels, petri dishes or TLC films, are placed onto the transilluminator surface. After the access doors are closed to prevent operator exposure to UV light, the lamp is powered on and the samples are photo-documented through the CCD or CMOS camera. Images are saved to the onboard software system for easy retrieval or export to Microsoft Excel or LIMS.
凝胶成像仪在哪里？
常见的分子生物学，基因组学，蛋白质组学和微生物学实验室，凝胶成像系统支持PCR段鉴定，DNA定量，细菌细胞培养，环境样品检测和蛋白质分离。
A - 凝胶成像仪波长频道
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Gel imagers包含过敏器that emit ultraviolet light or visible light for visualization of fluorescent and chemiluminescent samples or counting bacterial colonies. The UV or visible light bandwidth emitted by the transilluminator must match the absorbance spectrum of the fluorescent dye used during gel separation, blotting or thin layer chromatography.
Blue light, emitted at 470 nanometers, is optimal for colony counting or viewing samples stained with commercial dyes such as SYBR Green, SYBR Gold or SYBR Safe.
For UV visualization, 254 nanometer light is optimal for DNA cross-linking, 302 nanometer light is ideal for short exposuregels stained with Ethidium Bromide那and 365 nanometer light is best for gel band cutting.
B - GEL成像器使用和应用
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B1 - DNA凝胶
Nucleic acid (DNA or RNA)samples are loaded onto an agarose or acrylamide gel and exposed to an electrical field, causing the negatively-charged samples to travel down the gel toward the positive electrode. Smaller DNA or RNA fragments travel faster than longer fragments, allowing researchers to identify fragments tagged with fluorescent dyes to excise the samples for further purification. DNA gels are commonly used for PCR analysis, cloning, and next generation sequencing.
B2 - Protein Gels
Protein samples装载到浸没在市售缓冲液Tris-烯丙烯或三乙酸酯 - 三烯丙烯或三乙酸盐的聚丙烯酰胺凝胶上 - 用于分离和纯化。类似于DNA凝胶，不同长度的蛋白质片段通过凝胶基质以不同的速度迁移。蛋白质凝胶用于质谱，样品变性和印迹。
B3 - Colony Counting
殖民地计数is the process of determining the number of microbial colony forming units (CFUs) present within the optimal growth conditions of a petri dish loaded with cell media.
什么是殖民地形成单位？
Each colony forming unit represents a gross estimate of the number of homogenous, viable cells growing on the plate. Colony counting is used to detect and quantify microbes in soil, water or food samples as well as identify unique cell lines for microbial cell culture research.
B4 - 薄层色谱
Thin layer chromatography(TLC) is a research technique used to separate the components of a mixture using a thin film supported by an inert backing. As the mixture moves through the column, or stationary phase, its components will migrate at different speeds based on their affinity to the column or solvent (mobile phase). Common stationary phases include silica gel, cellulose, and aluminum oxide. Common mobile phases include methanol, acetic acid, and ethyl acetate. TLC is used to quantify and qualify many substances, including lipids, carbohydrates, fatty acids, and pesticides.
B5 - Southern and Western Blot Gel
蛋白质印迹凝胶那used to separate proteins for further isolation and purification, come in two primary forms: native gels and SDS-PAGE gels. Native gels, used to study enzyme or protein complexes, separate proteins based on size and ionic charge. SDS-PAGE gels, used to study antibody affinity, separate proteins based on size by denaturing the samples using the detergent sodium dodecyl sulfate (SDS).
南方斑点凝胶那used to separate DNA fragments from blood or tissue samples using restriction enzyme digestion, come in two primary forms: polyacrylamide (PAGE) gels with urea and sodium dodecyl sulfate (SDS) gels with urea.
C - Gel Imager Camera Resolution
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Gel imagers with camerasinclude CCD or CMOS cameras with resolutions from 5 megapixels to 17.9 megapixels. The megapixel number represents the quantity of individual points within an image. A camera with a higher pixel count produces images with higher resolution, greater sensitivity, and better image clarity.
D - Gel Imager Voltage
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120-volt connections are suitable for standard laboratory power outlets in the United States.
208伏或240伏连接，欧洲大陆，需要较少的电流（电流）和较小的导体，而不是设计用于120伏的设备。
E - 特殊凝胶成像器功能
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E1 - Integral Printer
Although most commercially-available gel imaging systems offer optional external printers.
Analytik Jena的UVP Photodoc-it系统包括一体式打印机，可方便地打印高质量凝胶图像。
E2 - 侧门门
德国耶拿分析仪器公司的GelStudio那GelSoloandMultiDoc-It成像仪包括侧面进入门，便于装载和卸载凝胶或培养板。
E3 - PLC Touchscreen Gel Imagers
德国耶拿分析仪器公司的ChemStudio那GelStudioandGelSoloimagers include integrated color touchscreens for filter and illumination control. Templates and macros, within the onboard software system, are programmable to support customized workflows.
Where Can I Buy Laboratory Gel Imagers Online?
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