TrayMix S4 Features and Benefits
Four independent hybridization chambers. Each chamber accepts one substrate slide for a total of 4 substrates per unit
Chambers can be launched simultaneously or set to run different protocols via the user-friendly graphical user interface
60μl的示例可以介绍我nto each hybridization chamber through dedicated access port. Gaskets (black), on upper portion of the lid, are used to seal chambers
Close ports prior to hybridization to prevent evaporation. System automatically delivers solutions into the 50 μl hybridization chambers when unit is programmed and substrates are in place
TrayMix S4 Automated Hybridization Station Chaotic Advection Mixing
Chaotic advection mixingassures that all biomolecules in solution reaches every spot on the microarray viamicrofluidic pumps combined with a mixing loop. The extremely complex direction and speed of fluids created by the mixing loop creates a chaotic movement, a very efficient way to mix low volume liquids in the shortest period of time. The chaotic advection mixing method in the TrayMix S4 accelerates hybridization kinetics by up to 90% and increases sample yield.
Static versus Dynamic Microarray Hybridization
| Probe concentration (0.1µM) |
Solution volume(µl) | Probe quantity (pmoles) |
Method | Hybridization solution | Fluorescent Mean (a.u.) | CV |
|---|---|---|---|---|---|---|
| Static Hybridization | 50 | 5 | cover slip | homogenous | 5500 | 0.33 |
| 500 | 50 | TrayMix™ S4 | homogenous | 38500 | 0.56 | |
| Dynamic Hybridization | 500 | 50 | TrayMix™ S4 | homogenous | 11474 | 0.18 |
| 500 | 50 | TrayMix™ S4 | non homogeneous | 11823 | 0.17 | |
| Average fluorescence values and coefficients of variation (CVs) measured under different hybridization conditions (2 hours of hybridization). | ||||||
| Probe quantity (5 pmole) |
Solution volume (µL) | Concentration (µM) | Method | Hybridization solution | Fluorescence mean (a.u.) | CV |
|---|---|---|---|---|---|---|
| Static hybridization | 50 | 0.1 | cover slip | Homogenous | 5500 | 0.33 |
| Dynamic hybridization | 500 | 0.01 | TrayMix™ S4 | Non-homogeneous | 6452 | 0.16 |
| Average value for fluorescence and CV obtained with the same quantity of targets, both with and without agitation (2 hours of hybridization). | ||||||
Dynamic advection mixing provides superior signal-to-noise ratios for microarray analysis. This image shows data obtained from two microarrays hybridized for two hours with a single-stranded CY3 labeled DNA probe complementary to allele a. Hybridizations were performed with 5 pmol of target under the traditional coverslip method and with the TrayMix™. All spot features were analyzed and compared to a reference background signal. Results comparing the mean signal to noise ratio of the fluorescent CY3 signals to the local background for allele a (black) and allele b (grey) of three experiments ± SD (Standard Deviation). These data show that the specific signal/noise ratio (allele a – black bars) is enhanced from 3.5 to 4.2, while the non-specific signal/noise ratio (allele b – grey bars) is slightly reduced under the dynamic hybridization conditions of the TrayMix S4.Software Interface
The easy software interface of the TrayMix S4 streamlines and easily define the hybridization process. The software interface has three main functions:- Traces operations and generates reports in HTML format
- Saves and loads protocols
- Modifies system operation in all of the experimental steps including pre-hybridization, hybridization and washing
Hybridization Protocol of TrayMix S4
- Set Pre-hybridization Time and Temperature
- Set Hybridization Time and Temperature
- Set Wash Parameters, program is ready to run!
- Insert microarray slide and click proceed
- Check the system is locked properly and port cap is properly closed.
- Programmed Pre-hybridization Program Starts, gets the microarray at proper temperature and wets the microarray prior to adding the labeled sample
- After complete Pre-hybridization and temperature is stabilized, remove air bubbles for the chaotic advection mixing-loop.
- Air bubbles are automatically removed.
- Inject sample with a volume of 30ul or 60ul, set volume and click proceed.
- The mixing loop automatically compensates for the volume injected for hybridization.
- Hybridization proceeds at set time and temperature with constant mixing.
- Wash steps proceed automatically according to set program parameters.
- When chamber is empty, microarray is removed from the system and dried in a microarray centrifuge.
